The UltraScence Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The UltraScence Western Substrate is designed for the detection of the target proteins in different amounts. Such as the low femtogram detection (mid-atto) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

• No need for optimization.

UltraScence Western ECL Substrate switching to other brands such as Pierce ECL and GE Healthcare does not require optimization or change of protocols.

• High sensitivity and enhanced chemiluminescence duration.

UltraScence Western ECL Substrate allows accurate low-picking or high-fly detection on the same immunoblot after a single exposure.

• Optimized use of PVDF and nitrocellulose membranes

• Compatible with Western blot markers.

• Optimized for film- and CCD-based imaging.